LPLC Multi-Column 2PC
Double capacity, halve the cost
Our multi-column chromatography systems offer significant advantages to those who need to increase capacity (de-bottleneck downstream) or reduce Protein A cost. Our EcoPrime Twin “2 PCC” solution is built on ChromaCon intelligence and patents and helps you take advantage of the most simple GMP scale multi-column technology.
When compared to conventional batch processes, the system delivers enhanced resin capacity utilization (up to 3X), decreased buffer consumption (by over 50% in many cases), and increased product concentration, all in a simple, two-column design that eases manufacturing validation and minimizes risk of down-time while reducing operating expense.
Productivity and simplicity:
- Using just two columns provides users with the lowest risk, simplest path to validation/qualification for regulated processes
- Increased productivity: The EcoPrime Twin LPLC running the CaptureSMB process delivers increased productivity (up to 3-fold) while both reducing resin costs and decreasing buffer consumption by up to 50% (or more).
- The risk of down-time and capital costs are minimized and operating expenses reduced when compared to other multi-column systems.
Process intensification and flexibility on a single skid:
EcoPrime Twin maximizes asset utilization, saves space and cost using pre-packed or conventional columns and options to have multiple modes of operation on single skid:
- Multi-column continuous or extended batch operation
- Conventional batch
- Sequential polishing (two different steps (e.g. bind/elute - flow through) with no hold up
- Buffer In-line Dilution capability integrated
Proven scale-up; scale down
- GMP customers have proven the direct scale-up / scale down with ChromaCon’s Contichrom CUBE. The fluidic design of the EcoPrime Twin LPLC system allows for direct scale-up or scale-down to the Contichrom CUBE FPLC from ChromaCon. Methods developed on the CUBE can be easily transferred to the EcoPrime Twin LPLC.
- Intuitive software for twin-column operation: The EcoPrime Twin LPLC automation and control software is designed to be user-friendly. Operators can easily navigate and manage process setup, execution, and reporting. The software is designed to enable 21 CFR Part 11 compliance.
- Advanced Pumps and Fluidics: The EcoPrime Twin LPLC system uses industry leading LEWA ecodos pumps and are enhanced with an exclusive digital pump control technology providing (1) wide flow-range accuracy for improved product yield and purity and (2) industry leading precision and batch-to-batch reproducibility.
To guarantee consistent and reproducible performance, the EcoPrime Twin LPLC system is designed with minimal holdup volumes and symmetric flow paths between the two columns, which was accomplished by optimal piping and components arrangements and sizing.
Simple, elegant and include:
- Pump(s) with hygienic design capable to deliver industry best-in-class precise flow.
- Multiple inlet and outlet valves.
- Air sensors to protect the columns or prevent dry running conditions.
- pH, conductivity, pressure and temperature probes/sensors
- Coreolis flow meters to control and/or verify pump flow rates
- Dual wavelength UV monitors in the range at 280 and 300 nm
- All necessary tubing, valves, instruments, connections and safety devices for GMP process requirements.
- System automation and control with an easy to follow user interface